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Chromatography
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is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the original mixture depends on how strongly each component is attracted to the stationary phase. Substances th ....
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....ts in the sample mixture pass through the column at different rates due to differences in their partition behavior between the mobile phase and the stationary phase. The compounds are separated by collecting aliquots of the column effluent as a function of time.
Conventional Liquid is most commonly used in preparative scale work to purify and iso.... |
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Page count: 3 |
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