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Fluorescence Study Of Dyes
Beginning of paper
1. Introduction
Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluorescence spectra.
For fluorescence spectra, ....
Middle of paper
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Coumarin 1
C = 8 x10-5 mol dm -3
We obtained a spectrum with a maximum at 376.5nm at an absorbance of 1.4573, using Beer-Lambert's law, we deduced the molar decadic absorption coefficients.
Sodium fluorescein
C = 2.425 x10-5 mol dm -3
Here max was 500.5 nm at an absorbance of 2.0922
Summary of the results obtained.
Dye
m.... |
| Word count: 815 |
Page count: 3 |
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